Specifications

Description

Adenosine serves as a nucleotide. It also plays an important role in the regulation of blood flow to various organs through vasodilation. It is actively involved in biochemical processes, such as energy transfer as adenosine triphosphate and adenosine diphosphate. Further, it is used in medication specifically as an antiarrhythmic agent to treat a number of supraventricular tachycardia. It is used to promote thickening of hair for people with thinning hair.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Solubility
Soluble in water, ammonium hydroxide and dimethyl sulfoxide. Insoluble in ethanol.

Notes
Store in a cool place. Incompatible with strong oxidizing agents.

Specifications

Description

Genomic DNA was isolated from Jurkat E6.1 (human acute T-cell leukemia, clone E6.1) cells grown to confluency in RPMI media supplemented with 10% bovine fetal serum. Isolated genomic DNA was treated with CpG Methyltransferase, repurified and dissolved in TE buffer.≥98% CpG methylation level confirmed by bisulfite sequencing.

Thermo Scientific CpG Methylated Jurkat Genomic DNA is human male genomic DNA from Jurkat cells, enzymatically methylated with CpG Methyltransferase (M.SssI). Genomic DNA was isolated from Jurkat (human acute T-cell leukemia) cells grown to confluency in RPMI media supplemented with 10% bovine fetal serum. Isolated genomic DNA was treated with CpG Methyltransferase, repurified, and dissolved in TE buffer. ≥ 98% CpG methylation level confirmed by bisulfite sequencing.

Highlights

• Pure - DNA free from inhibitors, proteins, and RNA
• Convenient - ready to use 100 ng/μL concentration
• Compatible with downstream applications
• Complete methylation of cytosines in CpG dinucleotides
  Applications
• As controls in the following:
• Bisulfite sequencing
• Combined Bisulfite Restriction Analysis (COBRA)
• Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE)

A260/230 ratio: ≥ 2
A260/280 ratio:1.75 to 1.98
Hazardous: No

Description

CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

• Wide selection of CyDye™ fluors for protein labeling for use in ELISA & immunoprecipitation
• Labeling strategy based on protein characteristics
• Medium to high-throughput fluorescence imaging
• Ready-to-use labeled antibodies enable multiplexed assays
• Streptavidin-conjugated CyDye™ fluors for use in Flow-cytometry and In situ hybridization
• Linkage of Cy dyes allows labeled DNA to hybridize to other DNA
• DNA synthesis reagent

Amersham™ CyDye™ fluors are versatile fluorophores designed for a broad range of applications. The use of multicolor CyDye™ fluors enables multiplexing without crosstalk. In addition, the CyDye™ flours readily dissolve in aqueous buffers, which eliminates the need of organic solvents. The high purity and thorough QC testing of Amersham™ CyDye™ fluors generate high levels of chromophore and reactive dye content. Narrow excitation and emission bands result in discrete signals from each fluor, which together with minimal cross-talk contributes to high accuracy.

Specifications