Catalog number: 082469

Related applications: Chromatography

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Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules. This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.

The GlycanPac AXH-1 LC column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 LC column provides unparalleled capabilities for glycan separations.

Unique Glycan Selectivity Based on Charge, Size and Polarity

• Excellent resolution for both native and labeled glycans
• Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
• Compatible with fluorescence and MS detection methods
• High chromatographic efficiency and excellent column stability

GlycanPac AXH-1 LC columns are based on high-purity and spherical silica substrates in both 1.9 and 3µm sizes for UHPLC and standard HPLC applications, respectively. The various dimensions of GlycanPac AXH-1 LC columns meet the needs of your applications with different resolutions, throughput and instrument requirements.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Catalog number: 22103-052130

Related applications: Chromatography

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Thermo ScientificTM HyPURITYTM C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

Highly characterized, tested and validated C18 columns

Ordering Information:

Other column dimensions are also available. Please call Customer Service for more information.

Catalog number: CHIRALKIT3UM150

Related applications: Chromatography

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Separate a wide range of enantiomers with Thermo Scientific™ Hypersil™ Chiral HPLC columns, designed for separations of neutral, acidic and basic racemates in normal phase. These chiral columns can help ensure your purification needs are met by resolving racemic mixtures and potential contaminants so you can be confident in the purity of your target compound. To maximize screening efficiency, these columns can be ordered in kits which contain a column in each of our chiral phases. All columns are available in analytical and preparative scale formats.

Separate Enantiomers

Hypersil Chiral JT

• Best starting point for method development.
• Chemistry: Tris-(4-methylbenzoyl)-cellulose modified silica.
• USP L80.

Hypersil Chiral AT

• Highest generality of all chiral phases.
• No aromatic ring necessary.
• No inversion of elution order possible.
• Chemistry: Amylose tris-3,5-dimethylphenylcarbamate modified silica.
• USP L51.

Hypersil Chiral OT

• Highly versatile chiral phases.
• No aromatic ring necessary.
• No inversion of elution order possible.
• Chemistry: Cellulose tris-(3.5-dimethylphenyl-carbamate) modified silica.
• USP L40.

Chiral Column Kits

• Contain three columns, one of each Hypersil Chiral JT, Hypersil Chiral AT, and Hypersil Chiral OT.

HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

Customer Specific Orders

• There are many other Chiral Stationary Phases (CSP’S) available from Thermo Scientific including immobilized brush-type phases. If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

Catalog number: 60312-201

Related applications: Chromatography

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Clean-up and concentrate target analytes in minutes with Thermo Scientific™ HyperSep™ Online SPE Products. Available in several convenient formats, the columns utilize popular HyperSep SPE chemistries. When used with conventional HPLC systems and appropriate switching valves, they provide:

• Effective removal of contaminants, such as proteins, from samples
• Fast, effective clean-up and concentration of target compounds

HyperSep HPLC Columns for Online SPE combine SPE chemistries with traditional HPLC column fittings and hardware.

HyperSep HPLC Columns for Online SPE are available with the following HyperSep SPE chemistries:

• HyperSep Retain™ PEP — for retention of polar and nonpolar analytes
• HyperSep Retain-CX — for retention of basic and nonpolar analytes
• HyperSep Retain-AX — for retention of acidic and nonpolar analytes
• HyperSep Hypercarb™ — for retention of extremely polar analytes

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.