Protein-based Chiral HPLC Columns
CHIRALPAK® AGP has extremely broad applicability for the separation of chiral drugs.
Basic compounds: CHIRALPAK® AGP、CHIRALPAK® CBHAcids, Non-protolytic compounds: CHIRALPAK® AGP、CHIRALPAK® HSA
- CHIRALPAK® AGP has extremely broad applicability for the separation of chiral drugs.
- The protein-based columns function entirely in the reversed-phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values.
- Possibility to dynamically improve enantioseparation.
CHIRALPAK AGP® has extremely broad applicability for the separation of chiral drugs.
○The chiral selector is alpha1-acid glycoprotein (AGP). This very stable protein is immobilized onto spherical 5 micrometer silica particles.
The CHIRALPAK® AGP column separates enantiomers of an extremely broad range of drug compounds.
・Amine (primary, secondary, and tertiary)
・Acids (strong and weak)
・Nonprotolytes (amides, esters, alchohols, sulfoxides, etc.)
○The chiral selector is Cellobiohydrolase (CBH). This is a stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles.
○CHIRALPAK® CBH is used for the separation of enantiomers of basic drugs from many compound classes. CHIRALPAK® CBH separates preferentially compounds containing one or more basic nitrogen's together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).
○The chiral selector is human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles.
○CHIRALPAK® HSA is especially suited for the separation of weak and strong acids, zwitterionic and nonprotolytic compounds.