Thermo Scientific™ CpG Methylated Jurkat Genomic DNA

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Thermo Scientific™ CpG Methylated Jurkat Genomic DNA

Thermo Scientific™ CpG Methylated Jurkat Genomic DNA main image
Thermo Scientific™ CpG Methylated Jurkat Genomic DNA-image

Description

Genomic DNA was isolated from Jurkat E6.1 (human acute T-cell leukemia, clone E6.1) cells grown to confluency in RPMI media supplemented with 10% bovine fetal serum. Isolated genomic DNA was treated with CpG Methyltransferase, repurified and dissolved in TE buffer.≥98% CpG methylation level confirmed by bisulfite sequencing.

Thermo Scientific CpG Methylated Jurkat Genomic DNA is human male genomic DNA from Jurkat cells, enzymatically methylated with CpG Methyltransferase (M.SssI). Genomic DNA was isolated from Jurkat (human acute T-cell leukemia) cells grown to confluency in RPMI media supplemented with 10% bovine fetal serum. Isolated genomic DNA was treated with CpG Methyltransferase, repurified, and dissolved in TE buffer. ≥ 98% CpG methylation level confirmed by bisulfite sequencing.

Highlights

  • Pure - DNA free from inhibitors, proteins, and RNA

  • Convenient - ready to use 100 ng/μL concentration

  • Compatible with downstream applications

  • Complete methylation of cytosines in CpG dinucleotides
    Applications

  • As controls in the following:

  • Bisulfite sequencing

  • Combined Bisulfite Restriction Analysis (COBRA)

  • Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE)

A260/230 ratio: ≥ 2
A260/280 ratio:1.75 to 1.98
Hazardous: No