BioPro HIC columns are capable of separating proteins under non-denaturing conditions. BioPro HIC HT offers a time-critical analysis of biopharmaceuticals such as antibody-drug conjugates (ADCs). BioPro HIC BF is useful for separation of especially low hydrophobic proteins.
Specification
Matrix | Bonded phase | Particle size (µm) | Usable pH range | Usable temperature range | Characteristics | |
---|---|---|---|---|---|---|
BioPro HIC HT | Hydrophilic non-porous polymer | Butyl group | 2.3 | 2-12 | 10-60°C | First-choice HIC column for high resolution/high throughput analyses |
BioPro HIC BF | 4 | HIC column for separation of low hydrophobic proteins |
Comparison of retention behavior
BioPro HIC HT (first choice column) is suitable for successful separation of various biopharmaceuticals.
BioPro HIC BF shows the stronger retention of proteins than BioPro HIC HT due to the stationary phase hydrophobicity, and could be preferred for the separation of low hydrophobic proteins.
Accura Triart is characterised by a bioinert surface coating on the column body and frit. It shows excellent peak shapes for highly adsorptive substances. Additionally, it prevents carry over and ensures complete recovery without column preconditioning. Therefore, it is especially ideal for the use in highly sensitive LC/MS analyses.
Accura Triart is suitable for analysis of nucleotides, proteins/peptides and metal-coordinating compounds.
Features
YMC-Triart materials filled in a bioinert surface coated hardware
Exceptional peak shapes with high sensitivities
Excellent recoveries without column preconditioning
Superior reproducibility and no carry-over effectsIdeal for highly sensitive LC/MS analyses
Meteoric Core is core-shell columns with outstanding resolution for UHPLC & HPLC. Meteoric Core can be used across a wide pH range and provides excellent peak shape for basic and coordination compounds compared to conventional columns or competitors'. This feature enables smoother method development. Meteoric Core is ideal for ultra fast and high resolution analysis. Meteoric Core can reduce its backpressure by half compared to sub-2 μm columns with the same resolution as this. Meteoric Core can be used with conventional HPLC as well as UHPLC.
Specification
Name | Meteoric Core C18 | Meteoric Core C18 BIO | Meteoric Core C8 |
---|---|---|---|
Base particle | Core-Shell type silica gel | ||
Particle size ( μm) | 2.7 | ||
Pore size (Å) | 80 | 160 | 80 |
Specific surface area (m2/g) | 150 | 90 | 150 |
Bonding | Trifunctional | ||
Carbon content (%) | 7 | 5 | 5 |
Endcapping | Yes | ||
pH range | 1.5-10 | 1.5-9 | |
USP Classification | L1 | L1 | L7 |
BioPro IEX Columns are for the separation of proteins, peptides, and nucleic acids. BioPro IEX Columns are available in QA and SP chemistries and are based on porous (QA and SP columns) and non-porous (QF and SF columns) hydrophilic polymer beads with low nonspecific adsorption. The porous materials offer excellent binding capacity with exceptionally high efficiency. The non-porous particles offer high efficiency and exceptional resolution.
POINTS
- Newly developed hydrophilic polymer beads with low nonspecific adsorption
- Effective surface structure designed to interact with biomolecules
- High theoretical plate number and symmetrical peak shape by optimal packing technology
- QA (quaternary ammonium) and SP (sulfopropyl) ion exchangers
- Ideal for analysis and laboratory-scale purification on porous type with high binding capacity and high recovery of biomolecules
- Ultra-fast analysis and high-resolution analysis on non-porous type